
簡要描述:Recombinant Mouse M-CSF ProteinFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine M-NFS-60 cells is less than 2 ng/ml
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Product Category詳細(xì)介紹
| 品牌 | absin | 純度 | > 95 % by SDS-PAGE and HPLC analyses. |
|---|---|---|---|
| 貨號 | abs00996 | 規(guī)格 | 10ug |
| 供貨周期 | 現(xiàn)貨 | 主要用途 | Lyophilized from a 0.2 μm filtered solu |
| 應(yīng)用領(lǐng)域 | 化工,生物產(chǎn)業(yè),農(nóng)林牧漁,制藥/生物制藥,綜合 |
Recombinant Mouse M-CSF Protein
| 概述 | |
| 別名 | M-CSF蛋白;CSF1蛋白 |
| 種屬 | Murine |
| 來源 | Escherichia coli. |
| 形態(tài) | Lyophilized from a 0.2 μm filtered solution in 20 mM Tris, 500 mM NaCl, pH 7.4. |
| 性能 | |
| 序列 | KEVSEHCSHM IGNGHLKVLQ QLIDSQMETS CQIAFEFVDQ EQLDDPVCYL KKAFFLVQDI IDETMRFKDN TPNANATERL QELSNNLNSC FTKDYEEQNK ACVRTFHETP LQLLEKIKNF FNETKNLLEK DWNIFTKNCN NSFAKCSSRD VVTKPDCNCL YPKATPSSDP ASASPHQPPA PSMAPLAGLA WDDSQRTEGS SLLPSELPLR IEDPGSAKQR PPRSTCQTLE |
| 標(biāo)簽 | No Tag |
| 純度 | > 95 % by SDS-PAGE and HPLC analyses. |
| 內(nèi)毒素水平 | Less than 1 EU/μg of rMuM-CSF as determined by LAL method. |
| 活性 | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine M-NFS-60 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg. |
| 溶解方法 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| 儲存/保存方法 | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 靶標(biāo) | |
| Accession | Q3U4F9 |
| 參考文獻(xiàn) | |
| 參考文獻(xiàn) | 1. Cosman D, Wignall J, Anderson D, et al. 1988. Behring Inst Mitt: 15-26. 2. Metcalf D, Willson T, Rossner M, et al. 1994. Growth Factors, 11: 145-52. 3. Hidaka T, Fujimura M, Nakashima A, et al. 2002. Jpn J Cancer Res, 93: 426-35. 4. Kubota Y, Takubo K, Shimizu T, et al. 2009. J Exp Med, 206: 1089-102. |
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