Rabbit anti-GAPDH Polyclonal Antibody

簡要描述：Rabbit anti-GAPDH Polyclonal Antibody的應(yīng)用WB: 1:5000-50000, IHC-F: 1:100-500, IHC-P: 1:100-500, IF: 1:100-500, ELISA: 1:5000-10000

產(chǎn)品型號：abs132004
廠商性質(zhì)：生產(chǎn)廠家
更新時間：2025-10-23
訪問量：2115

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品牌	absin	分子式	Predicted molecular weight: 38kDa
貨號	abs132004	規(guī)格	100ug
供貨周期	現(xiàn)貨	主要用途	WB: 1:5000-50000, IHC-F: 1:100-500, IHC-
應(yīng)用領(lǐng)域	化工,生物產(chǎn)業(yè),農(nóng)林牧漁,制藥/生物制藥,綜合

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概述
別名	GAPDH
宿主	Rabbit
反應(yīng)種屬	Human, Mouse, Rat, Hamster
應(yīng)用	WB: 1:5000-50000, IHC-F: 1:100-500, IHC-P: 1:100-500, IF: 1:100-500, ELISA: 1:5000-10000
分子量	Predicted molecular weight: 38kDa
免疫原	Recombinant human GAPDH full length protein
性能
形式	Liquid
濃度	1mg/mL
純化方法	affinity purified by Protein A
類型	Polyclonal Antibody
克隆號
同種型	IgG
儲存/保存方法	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
存儲溶液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
標(biāo)記	Unlabeled
修飾	Unmodified
靶標(biāo)
背景說明	oading Control Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36kDa subunits.
細(xì)胞定位	細(xì)胞骨架,細(xì)胞質(zhì),膜,細(xì)胞核
UniProt	P04406

研究領(lǐng)域
研究領(lǐng)域	Signal TransductionMetabolismEnergy Metabolism

實驗結(jié)果圖

Sample: Lane 1,4,7: Mouse Cerebellum tissue lysates Lane 2,5,8: Rat Cerebellum tissue lysates Lane 3,6,9: Human SH-SY5Y cell lysates Primary: Anti-GAPDH at 1/10000~1/100000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kDa Observed band size: 36 kDa

Sample: Lane 1: Cerebellum (Mouse) Lysate Lane 2: Raw264.7 (Mouse) Cell Lysate Lane 3: NIH/3T3 (Mouse) Cell Lysate Lane 4: Cerebrum (Rat) Lysate Lane 5: SH-SY5Y (Human) Cell Lysate Lane 6: MCF-7 (Human) Cell Lysate Primary: Anti-GAPDH at 1/10000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 38 kD

Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit,) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Liver ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

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