Rabbit anti-GAPDH Polyclonal Antibody

簡要描述：Rabbit anti-GAPDH Polyclonal Antibody,WB: 1:10000-200000, IHC-P: 1:100-500, IHC-F: 1:100-500, IF: 1:100-500

產(chǎn)品型號：abs124037
廠商性質(zhì)：生產(chǎn)廠家
更新時間：2025-10-29
訪問量：2000

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藥物陽性對照抗體磷酸化抗體二抗病毒抗體標(biāo)簽抗體內(nèi)參抗體其它一抗

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品牌	absin	分子量	37kDa
貨號	abs124037	規(guī)格	50uL
供貨周期	兩周	主要用途	WB: 1:10000-200000, IHC-P: 1:100-500,
應(yīng)用領(lǐng)域	化工,生物產(chǎn)業(yè),農(nóng)林牧漁,制藥/生物制藥,綜合

概述
別名	GAPDH抗體;GAPDH;Rabbit anti-GAPDH Polyclonal Antibody
宿主	Rabbit
特異性	GAPDH
反應(yīng)種屬	Human, Mouse, Rat, Rabbit, Porcine, Sheep, Goose, Chicken, Bovine, Pig, Rattus norvegicu, Duck, Broiler Chick, Cat, Zebrafish
應(yīng)用	WB: 1:10000-200000, IHC-P: 1:100-500, IHC-F: 1:100-500, IF: 1:100-500
分子量	Predicted molecular weight: 37kDa
免疫原	GAPDH protein of rabbit
性能
形式	Liquid
濃度	1mg/mL
純化方法	affinity purified by Protein A
類型	Polyclonal Antibody
同種型	IgG
儲存/保存方法	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
存儲溶液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
標(biāo)記	Unlabeled
修飾	Unmodified
靶標(biāo)
背景說明	Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 38kDa subunits.
翻譯后修飾	Acetylation
細(xì)胞定位	細(xì)胞骨架，細(xì)胞質(zhì)，膜，細(xì)胞核
UniProt	P04406

研究領(lǐng)域
研究領(lǐng)域	Signal TransductionMetabolismEnergy Metabolism

實驗結(jié)果圖

Sample:
Hela Cell (Human) Lysate at 40 ug
MCF-7 Cell (Human) Lysate at 40 ug
Primary: Anti- GAPDH (abs124037) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD

Sample:
Muscle (Rat) Lysate at 40 ug
Heart (Rat) Lysate at 40 ug
Primary: Anti- GAPDH (abs124037) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD

Sample: Muscle (Mouse) Lysate at 40 ug
Primary: Anti- GAPDH (abs124037) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD

Sample:
Thymus (Mouse) Lysate at 40 ug
Primary: Anti- GAPDH(abs124037)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer at 37°C for 30min; Antibody incubation with (GAPDH) Polyclonal Antibody, Unconjugated secondary primary antibody at 1:400 overnight at 4°C, and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer at 37°C for 30min; Antibody incubation with (GAPDH) Polyclonal Antibody, Unconjugated secondary primary antibody at 1:400 overnight at 4°C, and DAB staining.

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min;
Incubation: Anti-GAPDH Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min;
Incubation: Anti-GAPDH Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

Rabbit anti-GAPDH Polyclonal Antibody溫馨提示：本產(chǎn)品僅作科研實驗使用，不支持臨床等研究

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Rabbit anti-GAPDH Polyclonal Antibody

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