Rabbit anti-IL-1β Polyclonal Antibody

簡要描述：Rabbit anti-IL-1β Polyclonal Antibody，應(yīng)用FCM: 2ug/T，分子量Predicted molecular weight: 17kD/32kDa，免疫原KLH conjugated synthetic peptide derived from human IL-1 Beta:161-269/269

產(chǎn)品型號：abs126104
廠商性質(zhì)：生產(chǎn)廠家
更新時間：2025-10-28
訪問量：1860

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抗體

藥物陽性對照抗體磷酸化抗體二抗病毒抗體標(biāo)簽抗體內(nèi)參抗體其它一抗

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詳細(xì)介紹

品牌	absin	貨號	abs126104
規(guī)格	50uL;100uL	供貨周期	現(xiàn)貨
主要用途	FCM: 2ug/T

概述
別名	IL-1 β抗體;Catabolin; H1; IL-1β; Hematopoietin 1; IFN beta inducing factor; IL 1; IL 1 beta; IL 1B; IL1B; IL1F2; Interleukin 1 beta; Interleukin 1 beta precursor; LAF; OAF; Osteoclast activating factor; Preinterleukin beta; Pro interleukin 1 beta; IL1B_HUMAN; Interleukin-1 beta; IL-1 beta,IL1 beta
宿主	Rabbit
特異性	IL-1 Beta
反應(yīng)種屬	Human, Mouse, Rat
預(yù)測反應(yīng)種屬	Dog, Horse, Rabbit
應(yīng)用	WB: 1:500-2000, IHC-F: 1:100-500, IHC-P: 1:100-500, IF: 1:100-500, FCM: 2ug/Test
分子量	Predicted molecular weight: 17kD/32kDa
免疫原	KLH conjugated synthetic peptide derived from human IL-1 Beta(161-269/269)
Rabbit anti-IL-1β Polyclonal Antibody性能
形式	Liquid
濃度	1mg/mL
純化方法	affinity purified by Protein A
類型	Polyclonal Antibody
克隆號
同種型	IgG
儲存/保存方法	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
存儲溶液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
標(biāo)記	Unlabeled
修飾	Unmodified
靶標(biāo)
背景說明	The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008].
細(xì)胞定位	分泌型蛋白
UniProt	P01584

研究領(lǐng)域
研究領(lǐng)域	CardiovascularAtherosclerosisVascular InflammationInflammatory mediators ImmunologyInnate ImmunityCytokinesInterleukins MetabolismTypes of diseaseObesity NeuroscienceProcesses MicrobiologyOrganismVirusRNA VirusssRNA positive strand virusSARS Coronavirus

實驗結(jié)果圖

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-1 Beta) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Brain (Mouse) Lysate at 40 ug Intestine (Mouse) Lysate at 40 ug Primary: Anti-IL-1 Beta at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgGHRP at 1/5000 dilution Predicted band size: 17/30 kD Observed band size: 30 kD

Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-IL-1 Beta antibody Dilution: 2ug/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5ug/test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Sample: Raw264.7(Mouse) Cell Lysate at 30 ug Primary: Anti- IL-1 Beta at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17/30 kD Observed band size: 30 kD

Sample: Lane 1: Recombinant mouse IL-1b protein, C-His Primary: Anti-IL-1 Beta at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17/32 kDa Observed band size: 25 kDa

Rabbit anti-IL-1β Polyclonal Antibody溫馨提示：本產(chǎn)品僅作科研實驗使用，不支持臨床等研究

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Rabbit anti-IL-1β Polyclonal Antibody

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