Rabbit anti-KRAS Polyclonal Antibody

簡要描述：Rabbit anti-KRAS Polyclonal Antibody,WB:1:500-1:1000, IF/ICC:1:50-1:200, ELISA:Recommended starting concentration is 1 μg/mL.

產(chǎn)品型號：abs115469
廠商性質(zhì)：生產(chǎn)廠家
更新時間：2025-11-05
訪問量：2286

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品牌	absin	貨號	abs115469
規(guī)格	50uL	供貨周期	現(xiàn)貨
主要用途	WB:1:500-1:1000, IF/ICC:1:50-1:200	應用領(lǐng)域	化工,生物產(chǎn)業(yè),農(nóng)林牧漁,制藥/生物制藥,綜合

Rabbit anti-KRAS Polyclonal Antibody

概述
別名	KRAS抗體;NS; NS3; OES; CFC2; RALD; K-Ras; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; K-Ras 2; 'C-K-RAS; c-Ki-ras; c-Ki-ras2; KRAS,KRAS
宿主	Rabbit
反應種屬	Human, Mouse, Rat
應用	WB:1:500-1:1000, IF/ICC:1:50-1:200, ELISA:Recommended starting concentration is 1 μg/mL.
分子量	Detects a band of approximately 21kDa (Predicted molecular weight22kDa)
免疫原	A synthetic peptide corresponding to a sequence within amino acids 30-189 of human KRAS (NP_203524.1).
陽性對照	A-549,NIH/3T3,Raji,HeLa,Mouse spleen,Mouse brain,Mouse lung,Rat brain
性能
形式	Liquid
純化方法	Affinity purification
類型	Polyclonal Antibody
同種型	IgG
儲存/保存方法	Store at -20℃. Avoid freeze / thaw cycles.
存儲溶液	PBS with 0.05% proclin300,50% glycerol,pH7.3.
標記	Unlabeled
修飾	Unmodified
靶標
背景說明	This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.
細胞定位	Cell membrane,Cytoplasm,Cytoplasmic side,Lipid-anchor,cytosol
UniProt	P01116

實驗結(jié)果圖

Western blot analysis of various lysates using KRAS Rabbit pAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 60s.

Western blot analysis of lysates from C6 cells, using KRAS Rabbit pAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 180s.